The goal in this work was to increase yield by utilizing fiber chromatography for process compression. With speedto ‐ ‐ clinic being a competitive advantage and limited time available in contract
However, application of membrane chromatography has largely been restricted to low-resolution separation applications, primarily due to limitations associated with poorly designed
The incongruity of phage A5 encoding a chaperone with stronger tail fiber binding properties, but is also not required for producing functional tail fibers, suggests
In this study, we analyzed the tail fibers of published Acinetobacter phages and identified different functional domains present. These data will enable
Denyes JM, Dunne M, Steiner S et al (2017) Modified bacteriophage S16 long tail fiber proteins for rapid and specific immobilization and detection of Salmonella cells.
Optical fibers are routinely used in liquid chromatographic detectors as a means of simplifying optical designs. Selection of the appropriate fiber is an important factor in achieving
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An ideal sorting device should allow for both high sorting ef -fi ciency and reliable sorting consistency. Sorting ef ciency refers fi to the proportion of correctly sorted particles in relation to the total particles
Our data suggest that Pb2 not only forms the straight tail fiber but also fulfills the criteria of tape measure proteins. Furthermore, we show that its C-terminal region shares common features
This study sheds some light on the mechanism underlying the conversion of phage T5 straight tail fiber Pb2 from a phage-attached protein to a membrane-active
Fibersort® is a textile sorting machine that uses Near Infrared Spectroscopy (NIRS) scans to identify and separate textiles based on fiber composition. The machine uses AI models to predict the
This review highlights post-synthetic approaches for sorting single-walled carbon nanotubes — including selective chemistry, electrical breakdown,
RBPseg is a pipeline designed to predict and analyze phage tail fiber proteins. It has three major modules.
Abstract Tail-anchored (TA) proteins are bound to membranes by a hydrophobic sequence located very close to the C-terminus, followed by a short luminal polar region. Their active
In this work, 3D-printed microfluidic devices are designed and fabricated for optical chromatography and sorting. Optical chromatography is performed by inserting a single-mode optical
Herein, we established a new strategy to specifically separate and colorimetrically detect E. coli in water samples based on TFP-conjugated magnetic beads (TFP-MBs) and chromogenic
Fiber-based monoliths for use in liquid chromatographic separations are defined by columns packed with aligned fibers, woven matrices, or contiguous fiber structures capable of
Fiber length measurement As single fiber measurement takes time and hand stapling requires experience, alternative methods have been
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Explore the essentials of troubleshooting peak tailing, including pro tips, and suggestions for accurate and reliable chromatography.
(A) Organization of the tail fiber loci in bacteriophages T5, DT57C, DT57-1/2, DT571/2-ABF, and (partially) phiEco32. The similarity of the a.a. sequence regions in LTFs of T5-like phages
In this paper, we introduce RBPseg, a method that combines monomeric ESMfold predictions with a novel sigmoid distance pair (sDp) protein segmentation technique. This method
Despite the wide occurrence of Tfa proteins, their functional mechanism has not been elucidated. Here, we investigate the tail fibre and Tfa of Escherichia coli phage Mu.
A combination of all the above Peak shape changes are commonly observed in chromatography, and it is possible to quantitatively track peak shape over time. The causes of peak
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